Nail Pathogen qPCR Reagents
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Nail Pathogen qPCR Reagents

Part #: 0053|SKU: NP-4-10-T|Category: Reagents
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ISO 13485 certified

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Highlights

True Blue Bio Nail Pathogen qPCR Reagents are designed for rapid and accurate molecular detection of bacterial and fungal pathogens associated with nail and onychomycosis infections. Built on optimized real-time PCR chemistry, the assay delivers high sensitivity and specificity while integrating seamlessly into routine laboratory workflows.

The multiplex design enables simultaneous detection of clinically relevant nail pathogens along with key antimicrobial resistance markers in a single run. This comprehensive approach supports precise diagnosis, informed antifungal and antimicrobial therapy selection, and improved patient management.

The ready-to-use reagent format minimizes hands-on time and reduces technical variability, ensuring consistent and reproducible results across users and testing runs. Compatibility with standard real-time PCR instruments allows straightforward implementation within existing laboratory infrastructure.

Manufactured under stringent quality control standards, each reagent set undergoes comprehensive assay validation and lot-to-lot performance testing. These measures ensure reliable performance, high analytical accuracy, and minimal cross-reactivity in challenging keratinized nail specimens.

When paired with PCRopsis technology, True Blue Bio Nail Pathogen qPCR Reagents support flexible workflows, including extraction-free and direct-to-amplification options. These capabilities shorten turnaround times, lower operational costs, and enhance laboratory efficiency while maintaining strong analytical and clinical confidence.

Pathogen Coverage - 26

Bacteria-10

Fungus-12

AMR-4

Aerococcus urinae
Aspergillus fumigatus
Class A beta-lactamase resistance (CTX-M-Grp1)
Bacteroides fragilis
Aspergillus niger
Fluoroquinolone resistance (aac(6')-Ib-cr)
Klebsiella (Enterobacter) aerogenes
Aspergillus terreus
Methicillin resistance (mecA)
Klebsiella oxytoca
Candida albicans
Vancomycin resistance (vanA)
Morganella morganii
Candida glabrata
Proteus vulgaris
Candida parapsilosis
Pseudomonas aeruginosa
Candida tropicalis
Staphylococcus aureus
Epidermophyton floccosum
Streptococcus agalactiae (Group B)
Fusarium solani
Streptococcus pyogenes (Group A)
Trichophyton interdigitale
Trichophyton rubrum
Trichophyton spp. (rubrum/violaceum)

Technical Specifications

Formats:

  • 384-well pre-plated plate (full and partial filled options)
  • 96-well pre-plated plate
  • Pre-mixed, multiplexed tubes

Multiplex Mixes:

  • GRM-1 to GRM-8 (with internal control)

Platform Compatibility:

  • Applied Biosystems QuantStudio (v1.5.1+)
  • Applied Biosystems 7500 Fast Dx (SDS v1.4+)
  • Bio-Rad CFX96/CFX384 (Maestro v1.1+)
  • Other open qPCR platforms

For Research Use Only. Not for use in diagnostic procedures